rDNA Sequencing and Preliminary Analysis of Microorganisms from Diseased Edible Fungus Using Non-cul

• 作 者: Yao Qiang Gong Zhiyuan Gao Neng Han Jiandong Wan Luzhang Ren Pengfei Liu Xiao Huang Chunyan Ren Haixia
• 机 构: Institute of Agricultural Resources and Environment,Shandong Academy of Agricultural Sciences,Shando
• 出 处: Plant Diseases and Pests
• 发表日期: 2014年01期
• 关键词: edible sequenced Sequencing fungal Fungus alignment universal genomic DNA transf

The metagenomic DNA of disease tissue samples from four kinds of major edible fungus was extracted by CTAB method combined with DNA gel recovery kit. The genomic DNA was amplified by polymerase chain reaction using the universal primers of 16S rDNA and 18S rDNA,and then monoclonal sequenced after ligated and transformed. rDNA sequences of 20 positive clones were selected randomly from each pair of primers for sequence alignment. The results showed that there were two bacterial diseases and two fungal diseases in the samples,respectively.

The metagenomic DNA of disease tissue samples from four kinds of major edible fungus was extracted by CTAB method combined with DNA gel recovery kit. The genomic DNA was amplified by polymerase chain reaction using the universal primers of 16S rDNA and 18S rDNA, and then monoclonal sequenced after ligated and transformed. rDNA sequences of 20 positive clones were selected randomly from each pair of primers for sequence alignment. The results showed that there were two bacterial diseases and two fungal diseases in the samples, respectively.