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漆酶是降解森林凋落物中木质素的关键酶之一,直接影响着森林生态系统碳循环过程.运用TA克隆、测序技术,研究了两种亚热带森林(原生常绿落叶阔叶混交林和人工马尾松林)凋落物层(O层)和土壤表层(A层,0～20cm)降解木质素的担子菌漆酶基因多样性.结果表明:同一土壤层位,原生林土壤中担子菌漆酶基因多样性和种群丰富度高于马尾松林;同一森林生态系统,原生林土壤O层中担子菌漆酶基因多样性和种群丰富度略高于土壤A层,而马尾松林则O层明显低于A层;两森林土壤具有相同含漆酶基因的担子菌优势种群,且大部分优势种群与伞菌目小菇属或侧耳属有较高的氨基酸相似性;与原生林土壤A层和马尾松林土壤O层相比,原生林土壤O层和马尾松林土壤A层中含漆酶基因的担子菌种群分布相对均匀;马尾松林O层与A层之间漆酶基因核苷酸序列的相似性较原生林土壤O层与A层之间的高.表明植被和土壤层位显著影响漆酶基因多样性和群落结构,而植被和土壤层位引起的担子菌可利用底物和土壤pH值的差异可能直接驱动这种影响.
Laccase is one of the key enzymes to degrade lignin in forest litter, which directly affects the carbon cycle of forest ecosystem.Using TA cloning and sequencing technology, two subtropical forests (native evergreen and deciduous broad-leaved mixed forest and artificial Pinus massoniana) litter layer (O layer) and soil surface (A layer, 0 ~ 20cm) lignin degrading laccase laccase gene diversity.The results showed that: the same soil layer, the primary forest soil carrot laccase gene Diversity and population richness of Pinus massoniana forest were higher than those in Pinus massoniana forest. In the same forest ecosystem, the diversity and population richness of laccase laccase in O soil of the native forest were slightly higher than that of soil layer A, while that of Masson pine forest was significantly lower than that of A Layer. The dominant species of basidiomycetes with the same laccase gene were found in the two forest soils, and most of the dominant species had higher amino acid similarity with Agaricus or Pleurotus, Compared with the O layer, the distribution of basidiomycete population containing laccase gene in O soil layer and Pinus massoniana forest soil layer A was relatively uniform. The similarity of the laccase gene nucleotide sequence between O and A layer in Pinus massoniana forest Primary forest soil layer O and layer A. Between high, indicating that vegetation and soil horizon significantly affect laccase gene diversity and community structure, and vegetation and soil horizon due to differences in available Basidiomycetes substrate and soil pH may directly drive the impact.