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本研究发展了四种基于三羟基苯乙酮(THAP)的新型离子液体基质,即2′,3′,4′-THAP/二甲基苯胺(DMA)、2′,4′,6′-THAP/DMA、2′,3′,4′-THAP/吡啶(Py)及2′,4′,6′-THAP/Py,用于提高寡糖/糖肽在MALDI-TOF MS中的离子化效率.与传统的固体基质2,5-二羟基苯甲酸(DHB)和2′,4′,6′-三羟基苯乙酮(2′,4′,6′-THAP)相比,新型离子液体体系分析不同类型的寡糖链,均可获得更高的检测灵敏度.可使葡聚糖(dextran 1000)和环状寡聚糖β-环糊精在MALDI质谱中的信噪比提高10倍以上,RNase B的复杂寡糖链也实现了高灵敏度的检测.在糖肽分析中,2′,3′,4′-THAP/DMA离子液体高灵敏度地检测到辣根过氧化酶的7条糖肽,而2′,3′,4′-THAP作为基质时却无法检测到任何信号.
In this study, four novel ionic liquid matrices based on trihydroxyacetophenone (THAP) were developed, namely 2 ’, 3’, 4’-THAP / dimethylaniline (DMA), 2 ’, 4’, 6’- THAP / DMA, 2 ’, 3’, 4’-THAP / Pyridine and 2 ’, 4’, 6’-THAP / Py for improving the ionization of oligosaccharides / glycopeptides in MALDI-TOF MS Efficiency. Compared with the traditional solid substrates 2,5-dihydroxybenzoic acid (DHB) and 2 ’, 4’, 6’-trihydroxyacetophenone (2 ’, 4’, 6’-THAP) Liquid system analysis of different types of oligosaccharide chains, can obtain higher detection sensitivity can make dextran (dextran 1000) and cyclic oligosaccharide β-cyclodextrin in the MALDI mass spectrometry signal to noise ratio increased by 10 times , The complex oligosaccharide chain of RNase B also achieved high sensitivity detection.In the glycopeptide analysis, 2 ’, 3’, 4’-THAP / DMA ionic liquid highly sensitively detected 7 of horseradish peroxidase Glycopeptide, while 2 ’, 3’, 4’-THAP as a substrate can not detect any signal.