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目的：研究半边旗抗肿瘤有效成分６Ｆ对ＨＬ－６０细胞周期的影响及对常用抗肿瘤药的体外增效作用。方法：应用流式细胞光度术（ＦＣＭ）测定细胞周期，应用噻唑蓝（ＭＴＴ）法测定药物对细胞的抑制率。结果：不同浓度６Ｆ作用６小时即可使ＨＬ－６０细胞Ｓ期及Ｇ２／Ｍ期比例升高，Ｇ１期比例下降，并呈一定的剂量效应关系，当作用到２４小时后，Ｓ期比例进一步升高，但Ｇ２／Ｍ期比例稍有回落。低浓度６Ｆ分别与２－氯代脱氧腺苷（２－ＣＬｄＡｄｏ），顺铂（ＣＤＤＰ），长春新碱（ＶＣＲ），氟尿嘧啶（５ＦＵ）合用可增强它们对ＨＬ－６０细胞的杀伤作用，ｑ值大于０．８５，与各药有相加或协同作用，６Ｆ对２－ＣｌｄＡｄｏ，ＣＤＤＰ，ＶＣＲ，５ＦＵ的增效倍数分别为１．５８，１．５３，１．５５，１．３８。结论：６Ｆ可明显阻断ＨＬ－６０细胞在Ｓ期及Ｇ２／Ｍ期；６Ｆ可增强上述药物对ＨＬ－６０细胞的杀伤作用。已知，２－ＣｌｄＡｄｏ阻断细胞在Ｓ期，ＣＤＤＰ和ＶＣＲ阻断Ｇ２／Ｍ期，５ＦＵ阻断Ｇ１期。鉴于所试药物对细胞周期的影响不同，提示６Ｆ的体外增效作用可能与此有关。
Objective: To study the effect of 6F on the cell cycle of HL-60 cells and the synergistic effect of commonly used anti-tumor drugs in vitro. Methods: The cell cycle was determined by flow cytometry (FCM). The inhibitory rate of the drug on the cells was determined by MTT assay. Results: After treated with different concentrations of 6F for 6 hours, the proportion of S phase and G2 / M phase of HL-60 cells increased, the proportion of G1 phase decreased, and showed a dose-dependent effect. After 24 hours, the proportion of S phase was further increased Increased, but the proportion of G2 / M decreased slightly. Low concentration of 6F combined with 2-CLdAdo, CDDP, VCR and 5FU could enhance the cytotoxicity of HL-60 cells. The q value Greater than 0.85, with each drug additive or synergistic effect, 6F pairs of 2-CldAdo, CDDP, VCR, 5FU synergistic multiples were 1.58,1.53,1.55,1.38. CONCLUSION: 6F can obviously block HL-60 cells in S phase and G2 / M phase. 6F can enhance the cytotoxicity of HL-60 cells. It is known that 2-CldAdo blocks cells in S phase, CDDP and VCR block G2 / M phase, and 5FU blocks G1 phase. In view of the different effects of the tested drugs on the cell cycle, suggesting that 6F in vitro synergism may be related to this.