反相高效液相色谱法同时测定人血清中地尔硫卓及卡马西平浓度

• 作 者: 金克宁 张本全 饶度宁
• 机 构: 南昌铁路中心医院
• 出 处: 药物分析杂志
• 发表日期: 1994年03期
• 关键词: 地尔硫卓 药动学 日内精密度 二乙胺 钙通道阻滞剂 流动相 磷酸盐缓冲液 内标物 二次重蒸馏水 以安

本文采用反相高效液相色谱法，以安定为内标，同时测定血清中卡马西平（ＣＢＺ）和地尔硫（ＤＺ）的浓度。用Ｗａｔｅｒｓ产μ－ＢｏｎｄａｐａｋＣ_（１８）色谱柱，甲醇－ｐＨ为６．８４的磷酸盐缓冲液（内含０．０４％二乙胺）＝７０：３０（ｖ／ｖ）为流动相，紫外检测波长为２３７ｎｍ。ＣＢＺ两种浓度（５、１０μｇ／ｍｌ）的平均回收率及其日内精密度ＲＳＤ分别为１０１．３％、１０１．８％及０．７％、１．１％。ＤＺ两种浓度（１００、２００μｇ／ｍｌ）的平均回收率及其日内ＲＳＤ分别为１００．２％、９９．９％及２．７％、３．７％。本法较灵敏、准确、简单。可用于两药合用时血药浓度及药动学变化的研究。

In this study, reversed-phase high performance liquid chromatography with diazepam as internal standard, simultaneous determination of serum carbamazepine (CBZ) and diltiazem (DZ) concentrations. The mobile phase was eluted with μ-Bondapak C 18 (18) column, phosphate buffer (containing 0.04% diethylamine) = 70:30 (v / v) The wavelength is 237 nm. The mean recoveries of CBZ at two concentrations (5 and 10 μg / ml) and the intra-day precision RSD were 101.3%, 101.8% and 0.7%, 1.1%, respectively. The mean recoveries of DZ at two concentrations (100, 200 μg / ml) and their intra-day RSD were 100.2%, 99.9%, 2.7%, 3.7%, respectively. This law is more sensitive, accurate and simple. Can be used for the two drugs combined with plasma concentration and pharmacokinetic changes.