Inhibition of KU70 and KU80 by CRISPR interference,not NgAgo interference,increases the efficiency o

• 作 者: LI Guo-ling QUAN Rong WANG Hao
• 机 构: NationalEngineeringResearchCenterforBreedingSwineIndustry,WensFoodstuffGroupCo.
• 出 处: 农业科学学报:英文版
• 发表日期: 2019年2期
• 关键词: HOMOLOGOUS recombination non-homologous end-joining CRISPRi NgAgoi KU70 KU80
• 基金项目: the National Science and Technology Major Project for Breeding of New Transgenic Organisms,China(2016ZX08006002),the Guangdong Province“Flying Sail Program”Postdoctoral Foundation,China(2016).

Non-homologous end-joining(NHEJ) is a predominant pathway for the repair of DNA double-strand breaks(DSB). It inhibits the efficiency of homologous recombination(HR) by competing for DSB targets. To improve the efficiency of HR, multiple CRISPR interferen