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目的探讨小鼠匹鲁卡品癫痫持续状态模型(status epilepticus,SE)建立的优化给药策略。方法 98只ICR小鼠随机分为7组,每组14只,分别采用常规(一次量给药)或改良方法腹腔注射匹鲁卡品。改良方法为先给予150或200 mg·kg~(-1)匹鲁卡品,30 min内若未发作,则继续给予1~2次30~100 mg·kg~(-1)匹鲁卡品(给药时间间隔为30 min)。动物行为学结合海马脑电图用于评价SE成功诱导及动物猝死情况。结果常规方法:200 mg·kg~(-1)组(即一次量给予200 mg·kg~(-1)匹鲁卡品)造模成功4例,5例未SE发作,死亡5例;250 mg·kg~(-1)组造模成功3例,死亡11例;300 mg·kg~(-1)组动物全部死亡。改良方法:(150+50)mg·kg~(-1)改良组(先给予150 mg·kg~(-1)匹鲁卡品,若未发作则追加50 mg·kg~(-1)匹鲁卡品1~2次)造模成功6例,2例未SE发作,死亡6例;(150+100)mg·kg~(-1)改良组造模成功7例,死亡7例;(200+30)mg·kg~(-1)改良组造模成功6例,3例未SE发作,死亡5例;(200+50)mg·kg~(-1)改良组造模成功9例,死亡5例。结论常规一次量方法诱导小鼠SE模型时匹鲁卡品有效剂量较难控制,经改良后(200+50)mg·kg~(-1)的改良方法可能是小鼠匹鲁卡品SE模型建立的较优方法。
Objective To investigate the optimal drug delivery strategy of status epilepticus (SE) in mice. Methods 98 ICR mice were randomly divided into 7 groups with 14 rats in each group. The mice were injected intraperitoneally with pilocarpine by conventional (one dose administration) or modified method respectively. The improved method is to give 150 or 200 mg · kg -1 pilocarpine first, and to continue giving 30 to 100 mg · kg -1 pilocarpine within 30 min (Dosing interval is 30 min). Animal behavior combined with hippocampus electroencephalogram was used to evaluate the successful induction of SE and sudden death in animals. Results In the conventional method, 4 cases were successfully established in the 200 mg · kg -1 group (ie, 200 mg · kg -1 pilocarone), 5 cases were non-SE and 5 died. In the group of mg · kg ~ (-1), 3 cases were successfully established and 11 cases died. All the animals in the group of 300 mg · kg ~ (-1) died. Methods of improvement: (150 + 50) mg · kg ~ (-1) modified group (given 150 mg · kg -1 pilocarpine first, if no attack was added 50 mg · kg -1 Lukapo 1 ~ 2 times), 6 cases were successful in modeling, 6 cases were not attacked by SE, and 6 cases died. In the modified model group (150 + 100) mg · kg -1, 7 cases were successfully established and 7 cases died. 200 + 30) mg · kg ~ (-1) improved the model in 6 cases, 3 cases did not have SE attack, and 5 cases died. The rats in (200 + 50) mg · kg ~ (-1) , 5 died. CONCLUSION: The effective dose of pilocarpine can not be controlled when the conventional one-time method is used to induce mouse SE model. The modified method of (200 + 50) mg · kg -1 may be the model of pilocarpine SE Established a better method.