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目的了解临床分离的耐环丙沙星的大肠埃希菌质粒介导的喹诺酮类耐药基因的携带情况,并进行相关耐药机制的分析。方法采用VITEK-2全自动微生物检测系统鉴定细菌,用K-B法检测细菌对16种常用抗生素的敏感性,采用聚合酶链反应检测喹诺酮类耐药基因qnrS、qnrA、qnrB、qepA和aac(6′)-Ib,并对阳性的aac(6′)-Ib结果进行测序分析。结果 30株耐环丙沙星的大肠埃希菌中,2株(6.67%)检出qepA基因,8株(26.67%)检出aac(6′)-Ib基因,经测序证实其中6株为aac(6)′-Ib-cr(20.0%)。未检出qnrS、qnrA和qnrB基因。结论对环丙沙星耐药的大肠埃希菌携带aac(6′)-Ib-cr和qepA基因,引起质粒介导的对喹诺酮类抗菌药物的低水平耐药。
OBJECTIVE: To understand the carriage of quinolone resistance gene by Escherichia coli isolated from clinically isolated ciprofloxacin and analyze the mechanism of drug resistance. Methods The bacteria were identified by VITEK-2 automatic microbe detection system. The sensitivity of bacteria to 16 common antibiotics was detected by KB method. The quinolone resistance genes qnrS, qnrA, qnrB, qepA and aac (6 ’ ) -Ib, and the positive aac (6 ’) - Ib results were sequenced. Results Among the 30 Ciprofloxacin resistant Escherichia coli strains, 2 (6.67%) detected qepA gene and 8 (26.67%) detected aac (6 ’) - Ib gene. aac (6) ’- Ib-cr (20.0%). No qnrS, qnrA and qnrB genes were detected. Conclusion Escherichia coli resistant to ciprofloxacin carry aac (6 ’) - Ib-cr and qepA genes, resulting in plasmid-mediated low level of resistance to quinolone antibacterials.