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本文研究探讨了翡翠贻贝提取物对小鼠急性肝损伤的保护作用。建立四氯化碳(CCl4)致小鼠急性肝损伤模型,测定血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)含量及肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、肿瘤坏死因子α(TNF-α)和白介素-6(IL-6)含量;采用双向电泳分析肝脏差异表达蛋白;病理切片观察小鼠肝组织病理损伤的情况。结果表明,与模型组相比较,翡翠贻贝提取物各剂量组小鼠ALT、AST水平和MDA含量均显著降低,SOD、CAT、GSH-Px和GSH含量显著提高,TNF-α和IL-6水平也显著降低(p<0.01)。通过双向电泳获得了蛋白质显著差异点97个,鉴定出在模型组中有4个蛋白高表达,1个蛋白低表达;在翡翠贻贝提取物组中3个蛋白高表达。病理镜检显示小鼠肝脏损伤程度有所减轻。从以上实验结果可推测,翡翠贻贝提取物对CCl4引起的小鼠急性化学性肝损伤具有明显的保护作用。
This study explored the protection of Emerald mussel extract on acute liver injury in mice. Acute liver injury induced by carbon tetrachloride (CCl4) in mice was established and the levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and malondialdehyde (MDA) (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) Two-dimensional electrophoresis was used to analyze the differentially expressed proteins in liver. Pathological sections were used to observe the pathological changes of liver in mice. The results showed that compared with the model group, the ALT, AST levels and the content of MDA of the mice in each dose group were significantly decreased, the contents of SOD, CAT, GSH-Px and GSH were significantly increased, while the levels of TNF-α and IL-6 The levels were also significantly lower (p <0.01). 97 protein spots were identified by two-dimensional electrophoresis. Four proteins were found to be highly expressed in the model group and one protein was low, and three proteins were highly expressed in the extract group. The pathological examination showed that the degree of liver damage in mice was reduced. From the above experimental results it can be speculated that the extract of Juglans nigra has a significant protective effect on CCl4-induced acute chemical liver injury in mice.