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在海洋恶劣生态环境的压迫下,海洋微生物与海洋生物(如珊瑚、海绵等)形成了复杂的共生关系。这些海洋微生物在与其宿主的共生过程中,形成了自己独特的代途径,产生了许多结构新颖、活性显著的次级代谢产物。近年来,海洋来源微生物活性天然产物的研究已经成为了海洋药物研究的热点。本研究以三种东沙珊瑚共附生真菌Pseudallescheria boydii、Arthrinium sp.及Curvularia lunata.为研究对象,对其次级代谢产物进行了研究。通过正反相硅胶柱色谱、Sephadex LH-20凝胶柱层析、制备高效液相等多种现代色谱分离技术,从菌株发酵液的乙酸乙酯浸膏中共分离得到24个化合物。通过紫外,红外,一维二维核磁谱等多种现代波普技术并结合文献报道,鉴定了这些化合物的结构,包括大环内酯类化合物、萜类化合物、甾体类化合物等,其中新化合物6个。从第一种珊瑚共附生真菌Pseudallescheria boydii发酵液乙酸乙酯提取物中共分离得到13个化合物:(5S,6S,7R)-2,6,7-trimethyl-1,9-dimethylene-3-((2S,3S,Z)-3-methylhept-4-en-2-yl)-7-(1-phenylvinyl)spiro[4.4]non-2-ene(E-1)、2-((1S*,4S*,5S*)-4-hydroxy-4-(hydroxymethyl)bicyclo[3.1.0]hexan-1-yl)-6-methylhept-5-ene-1,2-diol(E-3)、(3S,5S,8S,10R,13R,17R)-10,13-dimethyl-17-((R,E)-6-methylhept-3-en-2-yl)-1,3,4,10,12,13,14,15,16,17-decahydro-2H-5,8-epidioxycyclopenta[a]phenanthren-3-ol(E-8)、(3S,5S,8S,10R,13R,17R)-10,13-dimethyl-17-((R,E)-6-methylhept-3-en-2-yl)-1,3,4,9,10,11,12,13,14,15,16,17-dodecahydro-2H-5,8-epidioxycyclopenta[a]phenanthren-3-ol(E-9)、(3S,4a R,5a R,9R,9a R,11b R)-9-((2R,5R,E)-5,6-dimethylhept-3-en-2-yl)-3-hydroxy-9a,11b-dimethyltetradecahydr ocyclopenta[1,2]phenanthro[8a,9-b]oxiren-6(5a H)-one(E-10)、(2S,3S,4R,4a R,5R,6R,8a R)-4,4a,6,8a-tetrahydroxy-5-methoxy-2,4-dimethyl-3-(3-methylbut-2-en-1-yl)decahydro-2λ3-isothiochromene-2-oxide(E-11),(2S,3S,4R,4a R,5S,8a R)-4,4a,8a-trihydroxy-5-methoxy-2,4-dimethyl-3-(3-methylbut-2-en-1-yl)octahydro-2l3-isothiochromen-6(2H)-one-2-oxide(E-12),(10R,13R)-17-((2R,5R,E)-5,6-dimethylhept-3-en-2-yl)-10,13-dimethyl-1,2,9,10,11,12,13,15,16,17-decahydro-3H-cyclopenta[a]phenanthren-3-one(E-22),(5R,6R)-5-(chloromethyl)-5-hydroxy-6-(hydroxymethyl)-6-((2S,3R)-2-methyl-3-(3-methylbut-2-en-1-yl)oxiran-2-yl)tetrahydro-2H-pyran-2-one(E-27),N-((4S,5R,7S,9S)-7-ethyl-9-hydroxy-8,8-dimethyl-2-oxo-1,6-dioxaspiro[4.5]decan-4-yl)acetamide(E-40),(1R)-4-methyl-1-(6-methyl-2-methylenebicyclo[3.1.1]heptan-6-yl)pent-3-en-1-ol(E-29),(3S,4R,4a R,5S,8a R)-4,4a,8a-trihydroxy-5-methoxy-4-methyl-3-(3-methylbut-2-en-1-yl)octahydro-6H-isothiochromen-6-one(E-51),(2S,3R,4R)-4-(chloromethyl)-3,4-dihydroxy-2-methoxy-3-((2S,3R)-2-methyl-3-(3-methylbut-2-en-1-yl)oxiran-2-yl)cyclohexan-1-one(E-63)。其中包括一些萜类和甾体类化合物。在这些萜类化合物中含有多种取代,有卤素氯取代的、硫取代的、磺酰基取代的,充分说明了微生物代谢途径的复杂性及独特性。化合物E-11,E-12以及E-51中两个六元环均为反式稠合。从第二种软珊瑚共附生真菌Arthrinium sp.发酵液乙酸乙酯提取物中共分离得到6个化合物:5-hydroxy-3-isopropyl-4-methoxyfuranone(B-1)、(S)-11,13-dihydroxy-4-methyl-4,5,6,7,8,9-hexahydro-2H-benzo[d][1]oxacyclododecine-2,10(1H)-dione(B-2)、24-Methylcholesta-7,22-diene-3β,5ɑ,6β-triol(B-3)、3β-hydroxy-5,9-epoxy-(22E,24R)-ergosta-7,22-dien-6-one(B-5)、(S)-3,4-Dihydro-8-hydroxy-3-methylisochromen-1-one(B-12)、3a,7-dihydroxy-6,6,9a-trimethyldecahydronaphtho[1,2-c]furan-3(1H)-one(B-21)。这些化合物均为已知化合物,包括大环内酯、甾体、萜类、聚酮等。从第三种软珊瑚共附生真菌Curvularia lunata.发酵液乙酸乙酯提取物中共分离得到5个化合物:(3S,5E,7S,8S,9S,11E)-7,8,9,14,16-pentahydroxy-3-methyl-3,4,7,8,9,10-hexahydro-1H-benzo[c][1]oxacyclotetradecin-1-one(C-1)、(3S,5E,7S,8S,9S,11E)-7,8,9,16-tetrahydroxy-14-methoxy-3-methyl-3,4,7,8,9,10-hexahydro-1H-benzo[c][1]oxacyclotetra decin-1-one(C-2)、(4b R,5a R,7S,8S,9S,13S,E)-1,3,7,8,9-pentahydroxy-13-methyl-4b,5a,6,7,8,9,12,13-octahydro-15H-benzo[c]oxireno[2,3-e][1]oxacyclotetradecin-15-one(C-3)、(3a S,5a R,8a R)-6-((2R,5R,E)-5,6-dimethylhept-3-en-2-yl)-3a-hydroxy-5a-methyl-3a,4,5,5a,6,7,8,8a-octahydro-2H-indeno[5,4-b]furan-2-one(C-4)、3-hydroxy-3-(2-oxopropyl)indolin-2-one(C-5)。这些化合物都是已知化合物,主要是大环内酯类化合物。本文首次对化合物C-1的核磁数据进行了报道。