不结球白菜霜霉病人工接种鉴定方法及抗性机制的研究

来源 :南京农业大学 | 被引量 : 0次 | 上传用户:liongliong497
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本文对不结球白菜霜霉病的人工接种鉴定、有关霜霉病抗性的生理生化机制及遗传模型进行了相关的研究,主要结果如下:1.利用不结球白菜的两个抗性自交系001、014和两个感病自交系007、010对不结球白菜子叶期和苗期的人工接种鉴定方法进行了研究,并对子叶期抗性、苗期抗性与成珠期抗性分别作了相关性分析,以确定子叶期和苗期人工接种鉴定的可靠性。结果表明:在子叶期,以接种浓度2×105个孢子/ml,接种量10μl/子叶的人工接种鉴定方法为最佳,能够准确鉴定出各个品种的抗感类型;在苗期,最佳的人工接种鉴定方法为接种浓度2×105个孢子/ml,接种量150μl/植株。根据成株期的鉴定结果及相关性分析得出,子叶期抗性与成株期抗性的相关性达到R2=0.8545,苗期抗性与成株期抗性的相关性达到R2=0.9027。2.利用4个不同霜霉病抗性的不结球白菜自交系,研究了苗期接种时,不结球白菜霜霉病抗性与几个生理生化特性的关系,旨在为不结球白菜的抗病品种的选育工作提供理论依据。结果表明:在接种病原菌前,不结球白菜健康叶片中的叶绿素含量和木质素含量与自交系对霜霉病的抗性成正相关,可溶性糖的含量与自交系对霜霉病的抗性成负相关,而SOD和几丁质酶与霜霉病抗性没有明显的相关性。接种后叶片中的叶绿素、木质素、可溶性糖、SOD和几丁质酶在抗感品种中均表现出明显的消长趋势,其动态变化与自交系对霜霉病的抗性一致。3.利用不结球白菜010×014、010×001两个组合F1、F2、P1、P2、BC1、BC2六个世代的材料,分别对不结球白菜霜霉病的子叶期抗性和苗期抗性进行了联合遗传分析。结果表明:两个组合霜霉病的子叶期抗性和苗期抗性均符合单基因显性遗传模型,且无母性遗传效应,因此在杂种优势利用的育种工作中,只需有一个亲本为抗性亲本即可,且抗性亲本既可做父本,也可做母本,这对新品种的育成非常有利。
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