论文部分内容阅读
To standardize the development of the leaf resources of Magnolia officinalis Rehd.et Wils.var.biloba Rehd.et Wils., the combination of simultaneous determination,chromatographic fingerprint, and hierarchical cluster analysis was used to evaluate the leaf quality.Five phenolic compounds isolated from the leaves were identified as rutin, afzelin,hyperoside, isoquercitrin, and quercetin-3-O-α-L-rhamnoside.Seven phenol compounds including honokiol and magnolol, were used as reference compounds to evaluate the leaf quality of seventeen samples of M.officinalis var.biloba collected from the different regions in China by high-performance liquid chromatography with photodiode array detection (HPLC-DAD) through a simple and reliable method of establishing simultaneous determination and chromatographic fingerprint.The calibration curves of seven compounds showed good linear regression (R > 0.9991) within test ranges.All RSD (relative standard deviations, %) values of linearity, stability, repeatability, precision, and recovery test were less than 3%.The recoveries of seven compounds were in the range of 97.1%-106.8%.Quercetin-3-O-α-L-rhamnoside and rutin were found to be predominant, their content range were 0.55-10.92mg/g and 0.34-5.56 mg/g.The phenol contents in the samples were relatively high in July when was suitable harvest time of the leaf.The eleven peak areas were used to evaluate the variation of chemical components by using hierarchical cluster analysis (HCA).The results showed that seventeen samples were classified Cluster Ⅰ, Ⅱ, Ⅲ, and Ⅳ.The combination of simultaneous determination, chromatographic fingerprint, and hierarchical cluster analysis can be readily utilized as a quality evaluation method for the leaf of M.officinalis var.biloba.