Molecular cloning and expression analysis of Epinephelus coioides TAK1 after Cryptocaryon irritans i

• 作 者: Xia Li Anxing Li
• 机 构: Key Laboratory for Aquatic Products Safety Department of the Ministry of Education/State Key Laborat
• 出 处: 中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会
• 发表日期: 2013年7期
• 关键词: TAK 1 Epinephelus coioide Cryptocaryon irritans

　　Cryptocaryon irritans a holotrichous ciliate is a marine fishe parasite,which can infect almost all kinds of marine fishe species and cause significant economic losses.Transforming growth factor:β activated kinas E-1 (TAK 1),a serine/threonine kinase in the mitogen:activated protein kinase kinase kinase (MAPKKK) family,plays an important role in the cascades of cellular responses aroused by changes in the environment.However,whether TAK1 participate in fish defense against C.irritans is poorly understood.In this study,we cloned the cDNA sequences ofEcTAK1,and found that the full length of cDNA sequences was 3568 bp,including a 5:untranslated region (UTR) of 272 bp,a 3:UTR of 1568 bp and an open reading frame (ORF) of 1728 bp which encods 575 amino acids.In expression analysis,the highest expression of EcTAK1 was observed in the blood,foliowed by the brain and the gill,and the expression of EcTAK1 did not differ significantly in other tissues.After in fection with C.irritans,we found that EcTAK1 expression was down:regulated in the skin at 6 h,1d and 3d; up: regulated in the head kidney at 1d and reached its peak,while the expression was significantly down:regulated at 3d and 5d.In the spleen,EcTAK1 expression was up:regulated at 6 h and reached its highest expression at 1d; and in the gill,it is evidently deduced at 6h and get to the highest expression level at day 2 but was not significantly different from that of the uninfected control group.In conclusion,the EcTAK1 expression can be regulated at different degree in different tissues after C.irritans infection,indicating that TAK1 is connected to the immuneesponse ofEpinephelus coioides.Further research should be carried out to elucidate the exact function of EcTA-K1 in E.coioides immunity in fish.