Simultaneous determination of aflatoxins and ochratoxin A in bee pollen by low-temperature fat preci

• 作 者: XiaoFengXue[1]LimingWu[2]YiLi;
• 出 处: 第十届海峡两岸蜜蜂与蜂产品学术研讨会暨首届全国蜂产业高峰论坛
• 发表日期: 2013年期

A simple and sensitive method has been developed for simultaneous determination of aflatoxins (AFB 1, AFB2, AFG1 and AFG2) and ochratoxin A (OTA) in bee pollen.The analytes in the sample were extracted with a mixture of acetonitrile/water (60:40, v/v), using low-temperature for fat precipitation, followed by immunoaffinity column clean up of extracts.The mycotoxins were quantified using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with an electrospray ionisation (ESI) and a triple quadrupole (QqQ) analyser.Matrix effect, accuracy and precision were evaluated and achieved good results calculated by matrix-matched calibration standards which reduced the influence of matrix effect.Recoveries at three levels were in the range of 74.3-96.5% with RSD less than 10.0%.The correlation coefficients (r2) of the five mycotoxins were higher than 0.997.The method showed high sensitivity with LOD below 0.05 μg/kg.