利用氧化石墨烯提高PCR扩增特异性研究

来源 :全国环境纳米技术及生物效应学术研讨会 | 被引量 : 0次 | 上传用户：cxg2009

【摘要】

：

PCR 是体外酶促合成特异DNA 片段的一种方法，不仅可用于基因分离、克隆和核酸序列分析等基础研究，还可用于疾病的诊断或需要进行DNA/RNA 分析的领域.PCR 扩增过程中存在假阴性、假阳性、非特异性扩增等问题，这些问题通常与PCR 扩增的特异性相关.研究表明氧化石墨烯(GO)能够与单链DNA 发生相互作用，影响其与互补链之间的相互作用.

【作者】

：

王园媛王丰邦宋茂勇汪海林

【机构】

：

中国科学院生态环境研究中心,北京 10085

【出处】

：

全国环境纳米技术及生物效应学术研讨会

【发表日期】

：

2016年7期

【关键词】

：

氧化石墨烯 PCR 特异性

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利用氧化石墨烯提高PCR扩增特异性研究

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