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Enclosed experimental system is used as a useful tool for manipulative experiment due to a greater degree of control and replication.Large scale mesocosm study could be one way of approach for merging the advantages of an enclosed incubation study and the benefits of a natural scale experiment.In order to investigate the effect of seawater CO2 concentration on the growth rate of natural assemblage of mixed phytoplankton,we designed and developed a mesocosm system including nine impermeable enclosures; each has a transparent cap,a CO2 concentration regulator,and a bubble-mediated seawater mixer.