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The main purpose of this work is to present a unique strategy in biomedical nanotechnology that allows simultaneous in vivo imaging and local therapy via hyperthermia.This novel concept is based on a kind of multifunctional Fe3O4 composite nanospheres consisting of polystyrene nanospheres (PS NSs,ca.100nm in diameter) fabricated with a narrow size distribution that are modified by polyethylene oxide and that contain Fe3O4 nanoparticles (5-10 nm) embedded in their matrices.