Effect of UCHL1 Epression and Localization on Testicular Development and Spermatogenesis of Chinese

• 作 者: HongJi
• 出 处: 第二届大鲵保护与发展论坛
• 发表日期: 2017年10期
• 关键词: Chinese Giant Salamander UCHL1 Male Gonad Development Extracellular Vesicles Ger

　　Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) which expresses extensively in vertebrate, is a deubiquitinating enzymes to inhibit degradation of protein by reversing ubiquitination modification.Here, a 1087 bp sequence encoding UCHL1 was identified from Chinese giant salamander (CGS, Andrias davidianus).The CDS of UCHL1 encoded a putative poly peptide of 222 amino acids.The CGS UCHL1 isoforms are more related to counterparts of human and mouse.The phylogenic tree of vertebrate UCHL1 indicates that CGS UCHL1 has the closest relationship with human UCHL1 (reach to 73.99%).Before gonad mature of male CGSs, the peak level of UCHL1 expression in testis appeared at the three-year-old CGSs by RT-qPCR and Western blot.In adult testis, the level of UCHL1 protein in testis tissues was lower in breeding period than in post-breeding period, while the level of UCHL1 protein in interstitial fluid of adult CGS testis was higher in breeding period than in post-breeding period.In testicular seminiferous Iobules at development stages of CGSs, immunohistochemistry displayed three kinds of localizing patterns of UCHL1 including nuclear localization at half year old, cytoplasmic localization from one year old to three years old, and extracellular localization in testis of adult CGSs.In testicular seminiferous Iobules of adult CGS, the different developmental germ cells were separated by niches containing UCHL1 protein, but UCHL1 did not localized on the mature sperm.The results displayed that the extracellular UCHL1 loaded on extracellular vesicles, as a component of the homogeneous germ cell niches, could regulate the synchronous development of sperm in testis of adult CGS.