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In this paper,a simple and efficient ultrasound-assisted reversed phase dispersive liquid–liquid microextraction(USARP-DLLME)coupled with ion-exchange chromatography(IC)/suppressed conductivity detection for the analysis of choline in edible oils was developed.Choline in the form of phosphatidylcholine(PC)in edible oils is micronutrient as well as degumming refinement remains affecting storage and quality.After hydrolyzing the hydrophobic compounds(PC)to water-soluble choline,free choline could be easily migrated into aqueous acidic phase.Several important parameters affecting hydroxylation and the extraction procedure has been optimized for the best recovery.Under the optimized conditions,the methods were validated through the establishment of the external standard calibration curves and the figures of merit.Detection limit(3S/N)of 0.012mg L-1in the range of 0.05-50mg L-1 with a correlation coefficient of 0.9997 for choline was achieved using the ion chromatography(IC)suppressed conductivity detection.The developed method was used for the quantification of the choline in edible oil samples of different varieties.