【摘 要】
：
A BmSG-SWU1 cell line was established from the silk gland tissues of newly hatched Bombyx mori larvae by performing primary cultures for two years.The cell line was comprised of long and thin shuttle
【机 构】
：
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China;Insti
【出 处】
：
中国遗传学会第九次全国会员代表大会暨学术研讨会
论文部分内容阅读
A BmSG-SWU1 cell line was established from the silk gland tissues of newly hatched Bombyx mori larvae by performing primary cultures for two years.The cell line was comprised of long and thin shuttle cells.The percentage of cells with the chromosome number 2n =56 was 76.28%.Therefore, the cell line was considered a diploid cell line.Fingerprint analysis of BmSG-SWU1 and four other cell lines indicated that BmSG-SWU1 had a specific fingerprint, and that the genetic relationship between BmSG-SWU1 and the original silk gland tissue was the closest.
其他文献
SRY是多数哺乳动物雄性性别决定基因,位于Y染色体.目的:对牦牛SRY基因的深入研究有望在分子水平上实现人为控制牦牛的性别,为今后遗传育种在分子方面奠定理论基础.方法:克隆测序SRY基因并利用生物信息学对其序列进行分析,利用DNAStar和CodonW分析软件分别研究麦洼牦牛、普通牛、绵羊、山羊、猪、小鼠、鸡和人该基因编码区的同源性及密码子偏好性.结果:麦洼牦牛SRY基因序列长721bp,含一个6
腓骨肌萎缩症(CMT)是一类较常见的遗传性周围神经病,群体发病率约为1/2500.根据临床和电生理特征,CMT主要分为两型:其中CMT1型以脱髓鞘为特征,CMT2型以轴突变性为特征.迄今,已报道有45个基因的突变与CMT的发生相关.课题组于2009年7月在山东省采集到一个CMT大家系,由52人组成,呈常染色体显性遗传.为了进一步揭示DHTKDI的生物学功能及导致CMT2Q的分子机制，课题组构建了D
牦牛是分布于青藏高原海拔3000-5500m,对高寒、缺氧、枯草期和冰封期等极端生态环境具有极强适应能力的特有畜种.动物线粒体DNA(mtDNA)具有结构简单、多拷贝、无重组、进化速度快、母系遗传、易扩增等优点,已成为分子进化研究中的好材料.本研究以西藏自治区的巴青牦牛、工布江达牦牛、江达牦牛、嘉黎牦牛、康布牦牛、帕里牦牛、斯布牦牛、桑日牦牛、桑桑牦牛、错那牦牛、丁青牦牛、类乌齐牦牛、隆子牦牛、聂
溶菌酶是动物体内十分重要的非特异性免疫因子,参与多种免疫防护反应,具有杀菌、抗病毒、提高免疫力、减轻炎症等多种功效,有着潜在的研究价值和商业前景,本实验室首次对牦牛胃溶菌酶基因进行了真核表达研究.参照GeneBank中牦牛胃溶菌酶基因的序列号(GenBank:EU780011.1),根据实验需要设计并合成引物.利用RT-PCR技术从牦牛胃组织总RNA中扩增获得特异性基因片段,将所获得的目的片段克隆
血友病B为凝血因子Ⅸ(FactorⅨ,FⅨ)基因突变或缺失导致的FⅨ蛋白功能异常或缺失引发的凝血功能障碍.传统上,血友病小鼠的模型构建采用同源重组的方法,该方法虽能大片段地造成基因缺失,抑制FⅨ活性,但其耗时长,筛选纯合小鼠难度高.CRISPR为RNA介导的靶向基因编辑系统,向导RNA(guideRNA,gRNA)可以通过与靶标DNA序列结合,形成三链结构,诱导核酸内切酶Cas9对DNA进行双链切
精原干细胞(SSCs)的自我更新与分化调控障碍是F1代雄性犏牛精子发生阻滞的主要因素之一.本研究运用新一代高通量测序技术(RNA-seq)对健康成年的牦牛和犏牛睾丸组织进行转录组测序,分析了两者中参与调控SSCs自我更新与分化相关基因的表达差异状况.结果表明:①Gdnf、Erm、Fgf2、Egf、Lhxl、Bcl6b、Ngn3、Plzf、Taf46等基因具有抑制SSCs分化或促进SSCs自我更新的
牦牛和普通牛的种间杂种犏牛雄性不育是牦牛杂交改良中的一大难题.本研究运用新一代高通量测序技术(RNA-seq)对健康成年牦牛和犏牛的睾丸组织进行转录组测序,分析两者睾丸组织中特异标记基因的表达差异状况.结果表明:①肌样细胞的标记基因,如Collagen4-a1和Collagen4-a2表达量在犏牛睾丸中显著上调,表明犏牛界膜中肌样细胞层显著增厚;②支持细胞的标记基因,如Vim、Shbg、Amh、F
Transcription factor SoxE is mainly expressed in gonad and involved in the regulation of gonad development and sex determination in animals.We based on silkworm ovary-derived cell line (BmN4-SID1) to
Caspases are a family of cysteinyl aspartate proteinases known for their essential roles in the regulation of apoptosis.To date, about 20 caspases have been identified although, few have been cloned a
The yellow-red color cocoon formation of Bombyx mori is due to the selective delivery of carotenoids from midgut lumen into silk gland, and is thought to be mediated by specific cellular carotenoids c