Simultaneous detection of Papaya ringspot virus, Papaya leaf distortion mosaic virus and Papaya mosa

• 作 者: P.He;W.Shen;D.Tuo;Y.Yang;P.Yan;X.Li;P.Zhou;
• 机 构: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture,Institute
• 出 处: 2014年度中国热带作物学会遗传育种专业委员会/热带香料饮料作物专业委员会年会暨学术研讨会
• 发表日期: 2014年期

　　Multiple viral infections are discovered in papaya cultivation.All three of the viruses included in this study caused significant economic losses in papaya farming worldwide.The development of a SYBR Green I based multiplex real-time PCR is described for simultaneous detection of Papaya ringspot virus (PRSV), Papaya leaf distortion mosaic virus (PLDMV) and Papaya mosaic virus (PapMV) in papaya.Members infected different virus were identified by their distinctive melting temperatures (Tms): 81.0 ± 0.8 ℃ for PRSV isolates, 84.7 ± 0.6 ℃ for PLDMV isolates, and 88.7 ± 0.4 ℃ for PapMV isolates.