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  5. Using EGFP-and DsRed2-Tagged Viruses to Study HIV-1 Drug Resistance,Replicative Fitness,and Tropism

Using EGFP-and DsRed2-Tagged Viruses to Study HIV-1 Drug Resistance,Replicative Fitness,and Tropism

来源 :2006中国天津世界艾滋病日科技会议 | 被引量 : 0次 | 上传用户：tlihao
【摘 要】
：
Numerous approaches have been used to evaluate HIV-1 drug resistance,replicative fitness,and/or tropism (co-receptor usage).More important,different assays are usually needed to quantify these charact
【作 者】
：
Miguel E.Quinones-Mateu 
【机 构】
：
Diagnostic Hybrids, Inc.Athens, OH, USA
【出 处】
：
2006中国天津世界艾滋病日科技会议
【发表日期】
：
2006年12期
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　　Numerous approaches have been used to evaluate HIV-1 drug resistance,replicative fitness,and/or tropism (co-receptor usage).More important,different assays are usually needed to quantify these characteristics in vitro.Here,we have developed and optimized a novel system cell-based assay which allows the simultaneous study of these viral parameters using dual-infections with EGFP/DsRed2-tagged viruses.HIV-1 replicative competent viruses were generated as fluorescent variants expressing EGFP or DsRED proteins in a NL4-3 genotypic background.
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