The possible mechanism of Ca2+ induced Ca2+ release in astrocytes

• 作 者: Jun-HuaYANG;Ke-QingZHU;Shu-MinDUAN;
• 机 构: Department of Neuroscience, School of Medicine Zhejiang University, Hangzhou 310058, China
• 出 处: 中国神经科学学会第九届全国学术会议暨第五届会员代表大会
• 发表日期: 2011年期

　　Objective Astrocytes are the primary neuronal structural and trophic supportive elements.Not like neurons which can generate action potential, however astrocytes exhibit diversity intracellur calcium activity and this is thought to play an important part in physiology and pathological conditions.Astrocytes calcium elevation has various forms,one important is Ca2+ induced Ca2+ release (CICR) which is thought to be mediated by 1,4,5-triphosphate receptor (IP3R) and ryanodine receptor (RyR) but the mechanism is not very clear.In our research, we want to explore the potential mechanism of CICR by a new way.Methods We used laser scan confocol microscopy to monitor the intraculler calcium variation and used a special method triggering Ca2+ influx in mouse astrocytes.The inhibitors of different channels were use to investigate the possible role of participating in CICR.Results Astrocytes calcium elevation was significantly inhibited by some specificity inhibitor.Conclusion Our primary datas showed that NCX may play an important role in astrocytes CICR.In contrary to some papers which reported RyR relating to CICR,our study didnt find this result.