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OBJECTIVE To investigate the effect of SBF on cortical cytoplasm apoptotic factors disturbances induced by complex Aβ25-35in rats.METHODS The cerebral injuries model was established by rats received intracere-broventricular injection of RHTGF-β1,Aβ25-35and Al Cl3and then accepted SBF treatment.All the rats were sacrificed by decapitation for indicators detection at last the drug treatment.Western blotting method was for caspase-3 protein expression and RT-PCR method detected cytochrome c,apoptotic protease activating factor-1(Apaf-1),caspase-9 m RNA expression in cortical cytoplasm.RESULTS The protein expression of caspase-3in cortical cytoplasm of rats was assayed by Western blotting.The results indicated that compared with the sham group,the caspase-3 protein expression of cortical cytoplasm in Aβgroup was significantly increased(P<0.01).However,the increased expression can be obviously reversed by SBF at doses of 35,70 and 140mg·kg-1,as compared with model group(P<0.01).The Cyt-C,Apaf-1 and caspase-9 m RNA expressions in cortical cytoplasm of rats were determined by RT-PCR.The results indicated that compared with the sham group,the Cyt-C Apaf-1 and caspase-9 m RNA relative expressions of cortical cytoplasm in Aβgroup was significantly increased(P<0.01).However,these increased expressions can be differently reversed by SBF at doses of 35,70 and 140 mg·kg-1,as compared with model group(P<0.01).CONCLUSION SBF can definitely improve rats′cortical cytoplasm apoptotic factors disorders induced by complex Aβ25-35,which maybe benefit for treatment of degenerative disease.
OBJECTIVE To investigate the effect of SBF on cortical cytoplasm apoptotic factors disturbances induced by complex Aβ25-35 in rats. METHHODS The cerebral injuries model was established by rats received intracere-broventricular injection of RHTGF-β1, Aβ25-35 and Al Cl3 and then accepted SBF treatment. All the rats were sacrificed by decapitation for indicators detection at last the drug treatment. Western blotting method was for caspase-3 protein expression and RT-PCR method detected cytochrome c, apoptotic protease activating factor-1 (Apaf-1), caspase-9 m RNA expression in cortical cytoplasm. RESULTS The protein expression of caspase-3 in cortical cytoplasm of rats was assayed by Western blotting. The results indicated that compared with the sham group, the caspase-3 protein expression of cortical cytoplasm in Aβgroup was significantly increased ( P <0.01) .However, the increased expression can be biblized by SBF at doses of 35, 70 and 140 mg · kg-1, compared with model group (P <0.01) .The Cyt -C, Apaf-1 and caspase-9 m RNA expressions in cortical cytoplasm of rats were determined by RT-PCR.The results indicated that compared with the sham group, the Cyt-C Apaf-1 and caspase-9 m RNA relative expressions Of cortical cytoplasm in Aβgroup was significantly increased (P <0.01) .However, these increased expressions can be orientated by SBF at doses of 35, 70 and 140 mg · kg -1, compared with model group (P <0.01). CONCLUSION SBF can definitely improve rats’cortical cytoplasm apoptotic factors disorders induced by complex Aβ25-35, which maybe benefit for treatment of degenerative disease.