花生中6个ERF家族蛋白基因的克隆及非生物胁迫下的表达模式分析

来源 :中国作物学会油料作物专业委员会第七次会员代表大会暨学术年会 | 被引量 : 0次 | 上传用户：cooltcp

【摘要】

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【作者】

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陈娜王冕禹山林杨庆利苏茂文潘丽娟迟晓元陈明娜和亚男杨珍王通

【机构】

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山东省花生研究所,山东青岛,266100 青岛市出入境检验检疫局,山东青岛,266001

【出处】

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中国作物学会油料作物专业委员会第七次会员代表大会暨学术年会

【发表日期】

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2013年12期

【关键词】

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花生 AhERF 表达分析非生物胁迫

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　　ERF家族蛋白在植物对低温、干旱及高盐胁迫的响应中具有重要的转录调控作用.然而花生中至今还没有有关该家族蛋白的报道.本研究从花生的cDNA文库中找到了40个编码AP2/ERF家族蛋白的EST序列,并对其中6个进行了基因全长的克隆.序列分析表明这6个基因编码的蛋白均只包含一个AP2/ERF保守结构域,属于ERF亚家族.6个基因分别命名为Arachis hypogaea ERF l-6(AhERF1-6).同时我们分析了这6个基因在低温、高盐和干旱胁迫下的表达模式.实验结果表明,AhERF4和AhERF6的表达对非生物胁迫具有快速明显的响应；AhERF1和AhERF5的转录在某些胁迫下略有提高.还有一些基因如盐处理叶片中AhERF3和干旱处理的叶片中AhERF2在胁迫下表达量有所降低.AhERF3和AhERF5则在花生干旱处理的叶片和根中表现出了相反的表达模式.以上结果表明不同的ERF蛋白在花生对非生物胁迫适应性中可能具有不同的功能.

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