Effect of Brn-4 on the neural stem cells differentiation into neurons

• 作 者: Jin-HongSHI;Guo-HuaJIN;
• 机 构: Medical School of Nantong University, Nantong 226001
• 出 处: 中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
• 发表日期: 2007年期

　　In order to investigate the effect of Brn-4 on the differentiation of the neural stem cells into neurons,we transected the fimbria of hippocampi of adult SD rats,then the extracts from fimbria transected and intact hippocampi were respectively obtained after the rats were breeding 14 days.Simultaneously the NSCs isolated from the hippocampus of embryonic rat were expanded and divided into 3 groups as following:(1) transection group:DMEM/F12 medium containing the extract of the fimbria-transected hippocampi was added; (2) normal group:DMEM/F12 medium containing the extract of the normal hippocampi was added; (3) control group:DMEM/F12 medium without any extract was added.The NSCs were cultivated and the neurons differentiated from the NSCs were detected by immunofluorescence doublelabeling of Brn-4 and MAP-2 on the 14th day after culturing.