Temperature-driven Metalloprotein-based Hybrid Hydrogels for Selective and Reversible Removal of Cad

• 作 者: Shanqing Huang Xichun Liu Qingyuan Hu Tianbiao Wei Chi Wu Hao Chen
• 机 构: State Key Laboratory of Coordination Chemistry,Nanjing University,Nanjing,210023
• 出 处: 第十一届全国化学生物学学术会议
• 发表日期: 2019年3期
• 关键词: thermosensitive hydrogel metalloprotein conformation change metal ion removal su

　　It is interesting to develop smart biomaterials for easily and reversibly removal of a trace amount of metal ions.1 Functionalized materials based on metalloproteins are very promising for the highly selective and efficient removal of heavy metal ions.Nevertheless,most developed protein materials typically recycle by releasing metal ions through chemical chelation,which limits their practical applications.We synthesized PNIPAM-co-CadRP,a thermally sensitive hybrid hydrogel by immobilizing a reconstituted cadmium binding peptide(CadRP)derived from metalloregulatory protein CadR in a poly(N-isopropylacrylamide)(PNIPAM)gel network.The thermally sensitive properties of the hybrid hydrogel,which swells at low temperatures(< 34 ℃)and shrinks at high temperatures,provides a driving force sufficient to alternate the conformation of the immobilized CadRP such that the peptide captures and releases metal ions at high and low temperatures,respectively.Using this novel hybrid gel,we successfully enriched nanomolar Cd(Ⅱ)from samples of environmental water in a highly efficient manner.This study showcases a practical and repeatedly reusable material for environmental remediation purposes and clinical diagnosis application that will significantly impact health and the environment.