Dolichos falcata Klein attenuated the inflammation induced by monosodium urate crystals in vivo and

• 作 者: LvyiChen;QinxiongLin;XukunDeng;XianjuHuang;ZhinanMei;
• 机 构: School of Pharmacy, South-Central University for Nationalities, Wuhan, 430074, P.R.China
• 出 处: 全国民族医药发展学术研讨会
• 发表日期: 2014年期

　　Ethnopharmacological relevance: Dolichosfalcata Klein (DF), a Chinese Dai ethnic medicine popularly known as "Tuoyeteng" in Yunnan province of China, has been widely used as a traditional herbal medicine for the treatment of fracture and beriberoid disease for a long time in China.Aim of study: The present study was carried out to investigate the components of DF, and to assess the anti-inflammatory activities of DF, as well as its possible mechanism on gouty arthritis in an animal model of the MSU crystals-induced gouty inflammation.Materials and methods: The ethanol extract (EE) of DF at the doses of 10, 20 and 40 mg/kg were administered to the rats treated by MSU crystals to evaluate the anti-gouty arthritis effect.Subsequently, the components of EE were isolated and identified using classical methods.Phyto-chemical analysis of EE was further carried out by HPLC-DAD.Finally, the anti-inflammatory effect of EE and two isolated components were assessed using the MSU crystals-treated monocyte/macrophage cell line RAW 264.7 in vitro.Results: EE (10, 20 and 40 mg/kg) significantly attenuated the pain threshold value, the joint swelling degree, the inflammatory cell infiltration of articular tissue and the increased levels of pro-inflammatory cytokines in MSU crystals-treated rats.Moreover, doliroside A and medicagenic acid-3-O-β-D-glucopyranoside were isolated and identified fiom EE.The major components of EE, including doliroside A, medicagenic acid-3-O-β-D-glucopyranoside and other triterpenoids, were well confirmed by HPLC.Further study revealed that EE, doliroside A and medicagenic acid-3-O-β-D-glucopyranoside (10, 20, 40 μg/mL) exhibited stronger inhibitory effects on the production of pro-inflammatory cytokines (interleukin-1β, interleukin-6 and tumor necrosis factor-α) in MSU crystals-treated RAW 264.7 cells.Conclusion: These findings indicate that the major saponins present in DF have remarkable effect on improving symptoms of acute gouty arthritis induced by MSU crystals through inhibiting the production of pro-inflammatory cytokines.