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目的观察黄芪甲苷对异丙肾上腺素(ISO)诱导的心肌损伤大鼠心肌组织中脂联素(adiponectin,APN)及其受体adipoR1的变化,探讨其作用机制。方法皮下注射异丙肾上腺素建立大鼠心肌缺血损伤模型,ELISA分析检测血浆及心肌组织中脂联素的含量,Real-time PCR方法检测心肌中脂联素及其受体adipoR,mRNA表达,Western blot方法检测心肌组织中adipo R1蛋白水平。结果与对照组相比,异丙肾上腺素组大鼠血浆和心肌组织脂联素含量明显降低,分别降低57.1和65.6%(均P<0.01),心肌脂联素及其受体adipoR1 mRNA的表达水平分别下降74.5%和60.1%(P<0.01);心肌组织中adipoR1蛋白水平明显降低。15 mg·kg-1·d-1黄芪甲苷治疗组大鼠血浆和心肌组织中脂联素较异丙肾上腺素组明显升高,分别增加94.1%和1.54倍(均P<0.01),心肌组织中脂联素及其受体adipoR1 mRNA的水平明显升高2.76和3.01倍(P<0.01),逆转异丙肾上腺素大鼠心肌组织中的adipoR1蛋白水平。异丙肾上腺素组大鼠血浆和心肌组织一氧化氮含量较对照组明显降低35.0%和74.8%(均P<0.01),15 mg·kg-1·d-1黄芪甲苷治疗组大鼠血浆和心肌组织中一氧化氮较异丙肾上腺素组分别增加68.6%和3.47倍(均P<0.01)。结论黄芪甲苷抗大鼠心肌损伤的机制可能与其上调心肌组织APN/adipoR1 mRNA和蛋白水平、增加一氧化氮生成有关。
Objective To investigate the changes of adiponectin (APN) and its receptor adipoR1 in rat myocardium induced by isoprenaline (ISO) and its mechanism. Methods The rat model of myocardial ischemia was established by subcutaneous injection of isoproterenol. The content of adiponectin in plasma and myocardium was detected by ELISA. The expression of adiponectin and its receptor adipoR mRNA in myocardium was detected by Real-time PCR. Western blot was used to detect the level of adipo R1 protein in myocardium. Results Compared with the control group, the content of adiponectin in plasma and myocardium of isoproterenol group decreased significantly (57.1% vs 65.6%, P <0.01), and the expression of adiponectin and its receptor adipoR1 mRNA The level of adipoR1 decreased significantly by 74.5% and 60.1% respectively (P <0.01). The levels of adiponectin in plasma and myocardial tissue of rats treated with 15 mg · kg-1 · d-1 astragaloside were significantly higher than those of isoproterenol group (94.1% and 1.54 times, respectively, P <0.01) Adiponectin and its receptor adipoR1 mRNA levels were significantly increased 2.76 and 3.01 times (P <0.01), reversed the level of adipoR1 protein in myocardial tissue of isoproterenol rats. Isoproterenol group rats plasma and myocardial nitric oxide levels were significantly lower than the control group 35.0% and 74.8% (all P <0.01), 15 mg · kg -1 · d -1 Astragaloside treatment group rats plasma And myocardial nitric oxide compared with isoproterenol increased 68.6% and 3.47 times (all P <0.01). Conclusion Astragaloside can inhibit the myocardial injury in rats possibly by up-regulating APN / adipoR1 mRNA and protein levels and increasing nitric oxide production.