Study on mutation in ALS of resistence to tribenuron-methyl in Myosoton aquaticum(L.) Moench

• 作 者: weitangliu;jinxinwang;taojin;yalingbi;lingxuli;guohuiyuan;
• 机 构: College of Plant Protection ,Shandong Agricultural University, Taian City, Shandong Prov.China
• 出 处: The 6th International Weed Science Congress(第六届国际杂草科学大会)
• 发表日期: 2012年期

　　[Background]Myosoton aquaticum(L.)Moench,a winter annual to biennial weed in the Pink family,can not be controlled by tribenuron after the herbicide has been continuously used for several years.The biotype ? collected from Zhumadian,Henan has emerged resistance to tribenuron-methyl and the resistance ratio up to 120.6 times.[Objective]To understand the molecular basis of the resistance mechanism to tribenuron-methyl in M.aquaticum(L.)and to find the specific mutation sites in amino acid sequence of acetolactate synthase(ALS)in the resistant biotype? of M.aquaticum(L.)[Method]Fragments encoding the ALS were amplified and cloned from M.aquaticum(L.),susceptible(S)and resistant ? biotypes to tribenuron-methyl,respectively,and sequenced subsequently.[Result]The result showed that the nucleotide sequence of R-biotype of M.aquaticum(L.)differed from that of the S biotype with one amino acid substitutions,of which,the amino acid substitution of Pro197 to Ser/Leu located in the highly conserved region Domain A.[Conclusion]The substitution of Pro197 might be responsible for the resistance to tribenuron-methyl in the R-biotype ofM.aauaticum(L.).