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Objectives: Try to map the Bjln2 gene as well as explore the relationship between Bjln1 and Bjln2.Methods: We hybrided the bilocular plants in NIL1 and NIL2 to produced F1,and then backcrossed the bilocular F1with multilocular to build BC1.The bilocular plants in F1 continuous self-cross to build the BC1.After assaying the segregation ratio of bilocular plants and multilocular plants in the population of BC1 and F2,andthe NIL2 for mapping the multilocular gene Bjln2.Combining the AFLP and BSA method with the comparing genomics to screen markers linked with theBjln2gene.A linkage map for multilocular gene Bjln2 was constructedusingthesemarkers.After sequencing about markers,we inferred thelocationofthe Bjln2 gene.Results: Assaying the segregation ratio of bilocular plants and multilocular plants in the population of BC1 and F2,BC1 population both have the 1:1 and 3:1 separation ratio; meanwhile,F2 population both have the 3:1 and 15:1 separation ratio,which can explain that the Bjln2 genewas not allelic to Bjln1 gene,the genotype of bilocular plants and multilocular plants in NIL2were Bjln1Bjln1BjLn2Bjln2 and Bjln1Bjln1Bjln2Bjln2 respectively.Thereare 20 markers(17 AFLP markers,an IP marker,a SSR marker and a SCAR marker)werefiltrated.The 20 markers were on both sides ofthe Bjln2gene.The closest markers on both sides were AL13and AL15etal,with genetic distance of 3.1 cM and 0.7 cM,respectively.Marker A1 was co-separated with Bjln2.Sequencingthe 17 AFLP markers,we inferred that Bjln2 was located in B genome.Combining with the rest of the three markers information,we hypothesized that multilocular genes Bjln2 may be located in B7 chromosome.Conclusions:Allelism analysis indicates that the genotype of bilocular plants and multilocular plants in the NIL2 was Bjln1Bjln1BjLn2Bjln2 and Bjln1Bjln1Bjln2Bjln2respectively.TheBjln2 gene was mapped in B7 chromosome.