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Clinical M.chelonae/abscessus complex strains collected from provinces in China were identified by phenotypic tests.Members were verified by Conventional biochemical tests and sequencing of 16s rRNA gene.Species and subspecies were identified by hsp65 and rpoB PCR-RFLP methods.In total,27 clinical specimens were identified as Mycobacterium chelonae/abscessus complex by phenotypic tests.16s rRNA gene sequence analysis of all 27 clinical samples shared over 99.7％ similarity with M.Chelonea and M.abscessus.Species identification with hsp65 PCR-RFLP and rpoB PCR-RFLP revealed that 18 specimens were M.abscessus and 4 were M.absecces,but the remaining 5 isolates displayed a pattern of rpoB PCR-RFLP that failed to match any previously reported pattern.