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Introduction Feather-sexable lines is widely used in poultry industry,but the phenomenon that the slow feathering are more sensitive to avian leucosis and more difficult to purify than fast feather was existed.The slow feathering (SF) gene linked with the ev21 gene which is an endogenous retrovirus (EV) incorporation may cause different immune effect to avian leukemia virus (ALV) infection. In this study, we intend to explore the differences of the innate immune response to ALV-infected between the slow and the fast feathering.