【摘 要】
：
The DNA modified gold nanoparticles (DNA-AuNPs) have been broadly used in the analytic chemistry.However,people found that the assembly efficiency of the DNA molecules on the AuNPs were very important
【机 构】
：
School of Basic Medical Science,Lanzhou University,Lanzhou,730000;Chemistry Departement,Tsinghua Uni
【出 处】
：
中国化学会第三届全国生物物理化学会议暨国际华人生物物理化学发展论坛
论文部分内容阅读
The DNA modified gold nanoparticles (DNA-AuNPs) have been broadly used in the analytic chemistry.However,people found that the assembly efficiency of the DNA molecules on the AuNPs were very important for the sensitivity of the DNA-AuNPs based sensors.Here,by extending a short duplex (12 bp) proximal to the AuNPs,we built a new kind of DNA-AuNP sensor (Figure 1).This sensor showed twice the sensitivity of similar sensors published previously.Meanwhile,when 30 nm AuNPs were applied in this sensor,the sensor could detect as low as 20 pM target DNA,this concentration was much lower than the limit of detection of the 50 nm sensor previously reported.It is foreseen that this strategy can be applied in many other sensors based on DNA-AuNPs.And more importantly,other signal amplification method could be further adopted on this sensor,which makes it possible to get better sensor system based on this kind of DNA-AuNps sensor.
其他文献
本文目的旨在同顾和总结1989-2013年我国实验用大小鼠肠道寄生虫病的流行现状，为实验动物生产、管理及使用者提供参考。结果 近年来我国清洁及以上大小鼠的寄生虫感染状况比过去有所缓解，部分地区SPF级实验动物的寄生虫感染率降到10％左右，但多数地区情况仍不容乐观。结论 实验动物质量是保证科学研究及检验准确性的重要基础，要进一步加强对实验动物寄生虫的防控，保证实验动物质量，适应科学研究、经济建设和社
目的 探讨屏障设施传递窗里紫外灯不同照射时间对实验物品的消毒效果.方法 将实验物品放置传递窗紫外灯照射15min、20min、30min利60min,采样检测物品表面的菌落总数和金黄色葡萄球菌、霉菌.结果 经过消毒液擦拭的签字笔、眼镜、百毒杀瓶和A4纸张放在四面有紫外灯管的传递窗中分别照射消毒15min、20min、30min和60min,细菌消亡率均可达到100％.结论 实验物品放置在四面有紫外
目的 建立武汉地区实验动物生理生化指标数据背景资料,为科学研究、实验结果评价提供科学、准确、客观的参考依据.方法 采用全自动生化分析仪及血液细胞分析仪对SPF级SD大鼠的血液生理生化指标进行测定,统计并分析雌雄个体间差异.结果 测定了SPF级SD大鼠血液生理生化指标数值,BA＃、EO％、BA％、ALP雌雄间差异显著(P＜0.05),其它差异不显著(P＞0.05).结论 比较客观的建立了SPF级SD
本文针对用户实际情况建立了一套基于分布式新能源的冷、热、热水三联产的系统。通过对当地太阳能资源以及负荷需求进行分析，对系统的方案进行了合理的设计。并对所需主要设备进行了选型，包括废气/空气源三用热同收机组，吸收式制冷机组，平板太阳能，槽式聚光太阳能以及中温高密度相变蓄热装置。系统主要功能包括三个部分：1)空调夏季制冷、冬季制热；2)动物楼四季供应生活热水；3)冬季制热设备的性能保障。对比分析了已有
本文针对作者单位的GLP动物设施系统设备特点，建立了一套安全、实用、经济、可控、可追溯的社会化技术服务体系；通过对湖北省食品药品安全评价中心GLP动物实验设施中央空调冷热源、净化通风空调、强弱电与送排水、电视监控与门禁、消毒、物流、节能及动物饲养工艺系统设备的社会化维护服务与专业化运行管理模式探讨，并在计划周全、SOP细致、记录完整、实时监督的服务手册指导下，通过近2年的实践检验，该设施系统设备得
环介导等温扩增(loop-mediated iso thermal amplification,LAMP)技术是日本学者Notomi等2000年建立核酸扩增技术,该技术能在恒温条件实现滚环扩增,扩增效率是PCR 10-1000倍,应用3对特异引物组合扩增,敏感性和特异性都比PCR高,与巢式PCR相当,且实验设备要求低,仅需一台恒温水浴锅就可以完成整个试验过程,特别适用于基层检验。另一方面,由于LA
Diatom analyses have been performed on a 1065 cm long Holocene undisturbed and finely laminated sediment sequence from Suminko Lake, Kashubian Lake District,Northern Poland.The absence of human activi
A general mean annual temperature increase has been recorded on the Yutman-Guizhou Plateau of China during the last century and most significantly since 1950s.To understand the response of lake ecosys
The largest array of Thrombolites (a type of Microbialites similar to the Stromatolites) in the world is located in Lake Clifton in Western Australia.Microbialites are believed to be built by a commun
Until now the main methods of water environment monitoring are physical and chemical methods.Although use these methods can measure characteristic and density of the special pollutant,it can only meas