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Background:Vascular dementia and ischemic stroke is a refractory disease,which is serious harm to human health and safety.The patented of prescription "WuShenXingNao" (WSXN) composing of the Radix fleece flower root,Ginseng and Ginkgo leaf,is candidate innovating drug for the treatment of ischemic stroke and vascular dementia,which was founded based on the Renshen Shouwu capsules and ginaton tablets and was based on the principle of treatment anti-cerebral ischemic injury neuroprotecting,promoting neuro-regeneration and improving learning and memory.Our previous study found that WSXN can significantly neuroprotect against cerebral ischemic injury,promote neuro-regeneration and improve vascular dementia,with the dual role of both prevention and treatment.Ginseng is an important part of WSXN.Now we study on the extraction method of ginseng total saponin,to establish the quality standards of ginseng extract (mainly for the ginsenosides).Objective:Studying on the extraction method of ginseng total saponin,preferred optimum extraction of ginseng ginseng total saponins to establish the quality standards of ginseng extract (mainly for the ginsenosides).Methods:Using the methods of water decoction extraction,ultrasonic extraction of water,ethanol refluxing extraction,extracts determination was used to the index in the extracts.Using L9 (34) orthogonal experiment design,in solvent concentration,solvent dosage,extraction time,extraction times to examine factors,high performance liquid chromatography were applied to determine the content of ginseng saponin Rgi and ginseng saponin Re,optimizing the best extraction process,Preparation according to optimum extraction of ginseng extract.Using HPLC method to determine the contents of ginsenoside Rgi,ginsenoside Re,ginsenoside Rbi and ginsenoside Rd in ginseng extract.Chromatography was perfomed on a Diamonsil-C18(150mm×4.6mm,5.0μm) at 300℃;the detection wavelength was 203nm;the mobile phase was composed of acetonitrile (A) and water (B) in gradient mode with a flow rate of l.OmL/min;injection volume was 10 μL.Results:The optimum way to extract ginsenosides was to reflux 80% ethanol eginseng in 12 volume of solvent ethano,for 1.5 hours every time,and Extracting 3 times.Adsorption resin D101 macroporous adsorption,eluted with 60% ethanol.On this condition,the content of ginseng saponin reached 61.85 %.Ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and ginsenoside Rd in ginseng extract get better separation and determination.The result of HPLC as follow:Ginsenoside Rgi showed good linearity in the range of 0.0579 μg-0.5808μg and the regression equation of Y=2002.5X-35219,(R2=0.9995),the average recovery was 99.53%,RSD =1.23% (n =6).Ginsenoside Re showed good linearity in the range of 0.0783 μ9-0.7827 μg and the regression equation of Y=3646X-2085.1,(R2=0.9996) the average recovery was 99.81%,RSD=1.28% (n =6).Ginsenoside Rbi showed good linearity in the range of 0.0302 μg~0.3019 μg and the regression equation of Y=5919.3X-25722 (R2=0.9994) the average recovery was 99.48%,RSD=1.14% (n =6).Ginsenoside Rd showed good linearity in the range of 0.0306 μg-0.3058μg and the regression equation of Y=9863.2X-23748,(R2=0.9998) the average recovery was 100.10%,RSD=1.82% (n =6).TLC result showed that ginseng extract present similar chromatography dots to ginseng.Conclusion:The optimum methods of ethanol refluxing extraction and preparation of macroporous resin adsorption separation and purification of high levels of ginseng total saponin extract is simple and feasible,operable,suitable for enterprise production.The method is rapid,simple,accurate,reproducible and is suitable for the determination of ginsenoside Rgl,ginsenoside Re,ginsenoside Rbi and ginsenoside Rd in ginseng extract,TLC is suitable for identification of the ginseng extract made from ginseng.