Protein-directed synthesis of highly monodispersed, spherical gold nanoparticles and their applicati

• 作 者: Yumin Leng Zhiwen Lu
• 机 构: College of Physics and Electronic Engineering, Nanyang Normal University, Nanyang 473061, China
• 出 处: 特种粉末冶金及复合材料制备/加工第一届学术会议
• 发表日期: 2016年11期
• 关键词: Proteins gold nanoparticles sensor array detection human tear

　　An in-situ reduction method has been reported to prepare gold nanoparticles (GNPs) of 40-110 nm by using the green reducing agents of proteins, which are activated by H2O2 and the superoxide anion (O2·-).The protein of collagen turns HAuCl4 to the aqueous Au(Ⅰ) ainions, which are further reduced by other proteins to be highly monodispersed and spherical GNPs of different sizes.The GNPs reduced by different proteins are found to be with the exposed {100} facets, the distinctive UV-vis absorption spectra and various colors.By means of extracting the color responses, such as red, green and blue (RGB) alterations, an in-situ reduction method-based multidimensional sensing platform is fabricated in the process of GNPs synthesis.Without further modification of GNPs, nine common proteins are found to be well detected and discriminated at different concentrations.Moreover, this sensing platform also demonstrates great potentials in qualitative and semiquantitative analysis on the individuals of these proteins with high sensitivity.Furthermore, the validation of this multidimensional sensing platform has been carried out by analysis on the spiked proteins in human urine and the targeted proteins in complex matrix (e.g.lysozyme in human tear).