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To clarifythe profile of neutralizing antibody (NAb) responses in HIV-1-infected individuals in China and epitope natures of viral envelope from broad neutralizers, the neutralizing ability of plasmas from chronic infected individuals from Xinjiang and Guangxi Province, against pseudoviruses of subtypes B, C, CRF07_BC, CRF01_AE and B using the TZM-b1 pseudovirus assay were analyzed.Breadth and strength of neutralization of the plasmas were evaluated.