MiR-21 regulates skin wound healing by targeting multiple aspects of the healing process

• 作 者: Tao Wang Yongping Su Zhongmin Zou Yimei Feng Huiqin Sun Lilong Zhang Lei Hao Chunmeng Shi Junping Wang Rong Li Xinze Ran
• 机 构: Institute of Combined Injury, State Key Laboratory of Trauma, Burn and Combined Injury, College of P
• 出 处: 第八届全国创伤修复(愈合)与组织再生学术交流会
• 发表日期: 2012年10期

　　With the clarification of the important roles of miRNAs in diverse physiological and pathological processes,the effect of miRNAs in wound healing becomes an increasing interest.However,the global pattern of miRNAs expression in wound tissue is still unknown.In present study,we depicted the miRNA profile and identified at least 54 miRNAs,including miR-21,changed for more than 2-fold at the stage of granulation formation during wound healing.These miRNAs were closely related to the major events of wound healing including cell migration and proliferation,angiogenesis,and matrix remolding.Furthermore,we found that miR-21 was upregulated after skin injury,mainly in activated and migrating epithelial cells of epidermis and mesenchymal cells of dermis.Locally antagonizing miR-21 by directly injecting antagomir to wound edge caused significant delay of wound closure with impaired reepithelialization and collagen deposition.Unexpectedly,we found wounds treated with miR-21 antagomir had an obvious defect in wound contraction at early stage of wound healing.The significant role of miR-21 in wound contraction was further confirmed by in vivo gain-of-function and in vitro loss-of-function experiments.In conclusion,present study has for the first time depicted miRNA profiling of wound healing and demonstrated the involvement of miR-21 in regulating the reepithelialization,wound contraction and collagen deposition.These results suggest that miR-21 may be a new medical target in skin wound manipulation.