【摘 要】
：
Cell free protein synthesis system(CFPS)can express protein in vitro without live cells.There are eukaryotic and prokaryotic cell free systems according to
【机 构】
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NationalUniversityofDefenseTechnology,Changsha,Hunan410073,China
【出 处】
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The 5th International Conference of Bionic Engineering 第五届国际
论文部分内容阅读
Cell free protein synthesis system(CFPS)can express protein in vitro without live cells.There are eukaryotic and prokaryotic cell free systems according to the origin of cell extract,of which E.coli cell extract system is the most common.CFPS can take DNA or mRNA as template,using energies and enzymes provided by cell extract to synthesis protein.CFPS has some advantages such as independence of live cells,costeffective and high-yield.Recently,CFPS is becoming a research hotspot in life science field.The basic principles are that PCR products or plasmids are transcribed into mRNA under the action of T7 RNA polymerase.The mRNA will be translated into protein with existence of ribosome,translational factors,amino acids and energies.mRNA will be released and join in next cycle.CFPS can efficiently express insect and vertebrate olfactory receptors,which is significant for bionic detecting based on olfactory receptors.
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