【摘 要】
：
Disulfide-rich peptides are emerging as potential templates for drug design applications.However,the precise pairing of disulfide-rich peptides is a logistical nightmare for chemists trying to modulat
【机 构】
：
College of Chemistry and Chemical Engineering,The MOE Key Laboratory of Spectrochemical Analysis and
【出 处】
：
2016年分析化学前沿国际研讨会及中美分析化学研讨会
论文部分内容阅读
Disulfide-rich peptides are emerging as potential templates for drug design applications.However,the precise pairing of disulfide-rich peptides is a logistical nightmare for chemists trying to modulate the folding pathway.The folding process of proteins is usually described as a black art.Nature gets around the oxidative folding challenge using various redox-active proteins,however this oxidative folding problem is still puzzling chemists.Thus,it would be interesting to develop artificial disulfide-rich peptide scaffolds possessing a predictive folding pathway,which will more broadly facilitate the use of disulfide-rich scaffolds for the design of stable peptide drugs.In theory,natural peptides with three disulfide bonds have 15 possible isomers.By rationally engineering the thiol-framework of a peptide containing six cysteines with penicillamine and dithiol amino acid,we demonstrated,for the first time,that the total number of isomers formed after the oxidative folding can be decreased to a minimal of two(Figure 1).Artificial scaffolds with an intrinsic and precise disulfide pairing propensity would be more tolerant to manipulation of primary sequence than natural disulfide-rich scaffolds without the augmented function.In addition,we envisioned that artificial scaffolds would also provide new insights into mechanism of oxidative peptide/protein folding.
其他文献
Microfluidic paper-based analytical devices(μPADs)represent a growing class of low-cost,user-friendly and disposable technology designed for point-of-use applications,which use porous cellulose to sto
Metallomics as an emerging scientific research field focuses on the science of biometal.These years we have been dedicating to analytical methodological development that is necessary to metallomics st
In the past dozen years,inductively coupled plasma mass spectrometry(ICP-MS)has emerged as a simple and spectacular tool for quantitative bioanalysis [1-5].We focused on the study of quantitative bioa
Persistent organic pollutants(POPs)are one class of environment pollutants that proved to be harmful to the environment and human health.They are causing ever-increasing concerns from scientists,the p
Thiocyanate(SCN-)is a well-studied spectro-electrochemical probe due to its simple structure and strong adsorption on the electrode surfaces.[1] Sum Frequency Generation spectroscopy(SFG)is a secondor
The ability to perform structure analysis of a molecule,such as the conformations of a protein,is critically important to understand its functions at a molecular level.Conventionally,tandem mass spect
Ultrasensitive and accurate detection of microbial pathogens is of critical importance for healthcare,food safety,water quality control,and antibioterrorism.Bacteriophages(or phages for short)are viru
Surface-enhanced Raman spectroscopy(SERS)is a surface-sensitive technique with ultrahigh sensitivity down to single-molecule detection [1].However,SERS suffers from the long-term limitation for chemic
In vitro culture of single cells facilitates biological studies by de-convoluting complications from cell population heterogeneity.However,there is still a lack of simple yet high-throughput methods t
Raman spectroscopy is one of the most powerful method in in-situ study of catalytic process due to its great advantage in low wavenumber detection as compared with infrared spectroscopy(IR).However,it