Studies on quantum dots and tetrahistidine tagged peptide in-capillary self-assembly using fluoresce

• 作 者: Jianhao Wang Jie Fan Li Liu Cheli Wang Jianpeng Wang Lin Qiu Pengju Jiang
• 机 构: School of Pharmaceutical Engineering and Life Science,Changzhou University,Changzhou,Jiangsu,China,2
• 出 处: 中国毒理学会纳米毒理学专业委员会第五届全国纳米生物效应与毒理学会议暨中国纳米医学会议2016(China Nanomed
• 发表日期: 2016年3期

　　● we utilized fluorescence coupled capillary electrophoresis(CE-FL)to probe the self-assembly between Cy5 labeled tetrahistidine tagged peptide(Cy5-A6G2H4)and CdSe/ZnS quantum dots(QDs)inside the capillary.● QDs and Cy5-A6G2H4 were injected into a capillary one after another and self-assembled inside the capillary.● Such in-capillary self-assembly was driven by a metal-affinity force which yielded a new fluorescence signal due to F(o)rster resonance energy transfer(FRET).● Under optimal conditions,a linear relationship between the integrated peak areas(S665/S605)and H6G6 was obtained.● The present assay is universal and promises potential applications in the online detection of biomolecules.