Disruption of a nitrogen regulatory gene AcareA in Acremonium chrysogenum leads to reduction of ceph

• 作 者: JinyangLi;yuanyuanPan;GangLiu;
• 机 构: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 10
• 出 处: 中国遗传学会第九次全国会员代表大会暨学术研讨会
• 发表日期: 2013年期

　　AcareA, encoding a homolog of the fungal nitrogen regulatory GATA zinc-finger binding protein, was cloned from Acremonium chrysogenum.Gene disruption and genetic complementation revealed that AcareA was required for nitrogen metabolism and cephalosporin C production.Disruption of AcareA resulted in growth defect in the medium using sodium nitrate, low concentration of ammonium chloride or glutamine as sole nitrogen source.In consistent with the reduction of cephalosporin C production, the transcription of pcbAB, cefD2, cefEF and cefG encoding the enzymes for cephalosporin C production, was reduced in the AcareA disruption mutant (△AcareA).