Heterodimerization of GHS-R1 and Orexin receptor 1 in living cells

• 作 者: Xue Qingjie Wang Chunmei Yang Chunqing Ji Bingyuan Pan Yanyou Chen Jing Bai Bo
• 机 构: 272067 济宁医学院神经生物学研究所
• 出 处: 第十八次全国行为医学学术会议
• 发表日期: 2016年5期
• 关键词: G protein-coupled receptor GHS-R1 heterodimerization resonance energy transfer O

　　Dimerization of G protein-coupled (GPCRs) is crucial for receptor function including agonist affinity,efficacy,trafficking and specificity of signal transduction,G protein coupling.The hypothalamus is the most important regulating region of brain and can express orexins neuropeptide.Through orexin receptors, orexins regulate arousal,wakefulness and play a critical role in drug addiction and reward-related behaviors.A lot of GHS-R1 is distributed in the anterior pituitary and central nervous system.GHSR is involved in the secretion of GH, the food intake and the accumulation of fat..Therefore, based on the research of GHS-R1 and Orexin receptor, we have been suggested that GHS-R 1 and Orexin receptor can form a functional hererodimer.Using BRET and FRET, we preliminarily provided conclusive evidence of heterodimerization beteen GHS-R1 and 0rexin receptor in a constitutive form.These novel data provide evidence for interaction of GHS-R1 and Orexin receptor and provide potential new pharmaceutical targets for obesity and other disease.