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Copper(Cu)is an essential heavy metal that is vital to the health of living systems.However,with the rapid development of industrialization,urbanization,and intensive agriculture in China,more and more agricultural soils have been contaminated with low-to-moderate Cu,and this has threatened the security of agricultural production,the natural environment,and human health.How to achieve the green,economic,and sustainable remediation of contaminated agricultural soils is still a topic of global concern.Screening of microbial species with high resistance to heavy metals has always been a frontier in the bioremediation of contaminated soils.This study presents environmental factors affecting copper removal by a newly isolated copper resistant fungi strain NT-1.The fungus resistant to high concentration of Cu(Ⅱ)was isolated from the decayed tissues of pumpkin treated with high concentration of copper sulfate,and was further identified as a Gibberella sp.by both the morphological characteristics of conidia and mycelial,and the 18S rRNA ITS gene sequencing analysis(100%similarity to Gibberella moniliformis and Gibberella fujikuroi).By using the single factorial design,the optimal growth and Cu(Ⅱ)removal conditions were determined as follows: temperature = 28 ℃,pH = 4.0,inoculation percentage 10%.Considerable growth of the isolate was observed at different concentrations of Cu(Ⅱ)varies from 100~500 mg L-1.Gibberella sp.NT-1 could remove nearly 100 mg L?1 Cu(Ⅱ)in water at 28 ℃,pH 4.0,in 5 days with the initial Cu(Ⅱ)concentration of 200 mg L?1 and initial inoculum of 20%.Results may provide evidence for the potential application of Gibberella sp.NT-1 in bioremediation of copper contaminated soil and wastewater.