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This study was undertaken to investigate the possible involvement of antioxidant defense and glyoxalase systems in protecting rice seedlings from heat-induced damages in presence of spermidine (Spd).Hydroponically grown 14-day-old seedlings were subjected to foliar spray with Spd (1 mM, 24 h) prior to heat stress (42 ℃, 48 h) followed by subsequent recovery (27 ℃, 48 h).