【摘 要】
：
响应面法已经能够运用于发酵过程的优化,可以对发酵培养基及培养条件等因素进行考察和评价.解淀粉芽孢杆菌对棉花黄萎病致病菌大丽轮枝菌具有明显的拮抗作用,为了探明生防菌株解淀粉芽孢杆菌的最佳发酵培养条件,提高其抑菌活性,应用响应面方法对该菌株发酵培养基进行优化,以抑菌圈半径为响应值.通过单因素多水平试验方法分析,研究碳源、氮源、C/N、培养温度、培养时间、转速、接种量、初始pH等因子对抑菌活性的影响,同
【机 构】
：
黑龙江八一农垦大学生命科学技术学院,大庆市 163319
【出 处】
：
第二十次全国环境微生物学学术研讨会
论文部分内容阅读
响应面法已经能够运用于发酵过程的优化,可以对发酵培养基及培养条件等因素进行考察和评价.解淀粉芽孢杆菌对棉花黄萎病致病菌大丽轮枝菌具有明显的拮抗作用,为了探明生防菌株解淀粉芽孢杆菌的最佳发酵培养条件,提高其抑菌活性,应用响应面方法对该菌株发酵培养基进行优化,以抑菌圈半径为响应值.通过单因素多水平试验方法分析,研究碳源、氮源、C/N、培养温度、培养时间、转速、接种量、初始pH等因子对抑菌活性的影响,同时采用响应面法分析培养条件对解淀粉芽孢杆菌W17抑菌活性的关键影响因素,确定最佳的产抑菌物培养基.结果 显示：在单因素最佳培养条件确定的基础上,通过Plackett-Burman实验筛选出影响菌体发酵的关键因素为C/N、培养温度和培养时间,通过最陡爬坡法和Box-Benhnken(响应面)分析法的最佳培养条件为培养温度34℃、C/N为1∶1、培养时间为12h,其它的最佳培养条件为pH7.5、转速为165 rpm、接种量为2％、碳源为葡萄糖、氮源为胰蛋白胨.在此培养条件下,优化后抑菌圈半径从试验设计初期的9 mm提高至14 mm,且经过验证试验证实预测值与实际值之间具有较好的拟合度.
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