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Objective To explore the feasibility of mediated HGF gene treating acute myocardial infarction by the combination of ultrasound microbubbles and TAT peptide.Methods Forty SD rats were randomly divided into 5 groups after the models of myocardial infarction were prepared, ①blanked control group (C), ②ultrasound+blanked microbubble group(US+MB), ③ultrasound+TAT-loaded microbubble group(US+MBTAT), ④uhrasound+ HGF-loaded microbubble group (US+MBHGF) and ⑤ultrasound+ HGF-TAT-loaded microbubble group (US+ MBHGF+TAT).All the rats were killed after gene transfection 7 days.Expression of the report gene EGFP in the rat myocardium was observed using laser confocal microscopy.Distribution of collogen in the rat myocardium was observed using polarizing microscopy.The CD34 expression was detected by IHC, and microvessel density (MVD) was deternined.Expression of HGF mRNA and HGF peptide was detected respectively by RT-PCR and Western Blot.Results Green fluorescence intensity of US+ MBHGF +TAT group was higher than that of other groups,its collogen was fewer than that of other groups, and its expression of HGF mRNA and HGF peptide was higher than that of other groups.IHC results showed that MVD of US+ MBHGF+TAT group was the highest among the groups.Conclusion The combination of ultrasound microbubbles and TAT peptide can effectively deliver HGF gene into infracted myocardium, promote angiogenesis and improve fibrosis, which can provide a novel strategy for gene therapy of ischemic heart disease.