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In the recent decades, there found overwhelming evidences showed that the climate is changing rapidly, which has been the research hotspot in the area of evolution and ecology.Global climate change is one of themajor environmental stressesthat threating biodiversity and the living of organisms, as a result, plant always get some phenotypic variation to adapt to the environment, however, for plants, the successful reproduction requires exact timing of flowering, and the time of flowering during the year is an important adaptive trait.Previous studies witnessed that the time of flowering in wild species have been earlier than before, showed that the change of flowering time is an effective strategy to adapt to the climate change.In 2012, a research examined 10 wild barley sampled in 1980 and again in 2008 detected profound adaptive changes in flowering time (Nevo et al., 2012 PNAS), the results found that the average flowering time of the all ten populations of wild barleyin 2008 have been distinct earlier than that in 1980, and SSR analysis showed that the frequencies of genotypes have been changed to mitigate the global climate change.However, thegenetic mechanism of the phenomena has not been detected.We examined the phenotypeof 10 wild barley populations sampled in 1980 and again in 2008, then took advantage of the genome of barley to investigate the nucleotide diversity and linkage disequilibriumof the flowering time networks based on the Sanger sequencing and Next-generation sequencing.Toanalyze the frequency and the co-contribution to the phenotypes of the regulatory network of flowering time genes,we used the clinal variation and candidate gene association study approaches to map the mutationswhich contribute to the complex adaptive traits.This study will address the genetic basis underline early flowering in natural populations of wild barley, to provide an genetic example how annual plantmitigate in a short generations to adaptthe global climate change by regulating flowering timefor the first time.