【摘 要】
：
In this study, on-line concentration-capillary electrophoresis was used to determine catecholamines.Different methods were employed namely, CE-UV and CE-LIF combined with cation-selective exhaustive i
【机 构】
：
Department of Chemistry, Chung Yuan Christian University (CYCU), 200 Chung Pei Road, Chung-Li 320, T
【出 处】
：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
论文部分内容阅读
In this study, on-line concentration-capillary electrophoresis was used to determine catecholamines.Different methods were employed namely, CE-UV and CE-LIF combined with cation-selective exhaustive injection and sweeping (CSEI-sweeping) and field-amplified sample stacking (FASS), respectively.In the CE-UV coupled with CSEI-sweeping method, separation conditions such as micelle and buffer concentrations and, on-line concentration conditions such as sample matrix and acid plug concentrations, water and acid plug length were optimized to find the suitable conditions for catecholamines determination.In the fluorescence detection of catecholamines, derivatization conditions such as derivatizing agent concentration, reaction time, buffer and pH value were optimized to find the best conditions prior to analysis.The above-mentioned two methods were used to test real samples such as biological samples.
其他文献
乙二醇是一种重要的有机化工原料,主要用于生产聚酯、汽车用冷冻剂等产品。紫外透光率(简称UV值)是衡量聚酯级乙二醇产品质量的一项重要指标。乙二醇UV值不合格,将影响下游产品聚酯的质量,如光泽和色度。研究影响乙二醇UV值的杂质(UV物)的结构及其成因,对于改进乙二醇生产工艺、提高产品质量具有积极的意义。由于影响乙二醇紫外透光率的杂质含量极低,使用气质联用技术或液相色谱手段难以直接进行结构鉴定。本文采用
The sol-gel technology is applied for the preparation of three solid-phase microextraction (SPME) fibers for extracting of the volatile organic compounds (VOCs) from environmental water samples using
同型半胱氨酸(homocysteine,Hcy)为一种含巯基的氨基酸,是甲硫氨酸的代谢中间产物.大量研究证实,Hcy与心脑血管疾病、肝脏疾病、肾脏疾病、老年痴呆症等多种疾病有关,是导致心脑血管疾病的一个独立危险致病因子.同型半胱氨酸浓度监测主要应用于临床冠状动脉粥样硬化和心肌梗塞患者,它的浓度升高程度与疾病的危险性成正比.半胱氨酸(cysteine,Cys)、半胱氨酰甘氨酸(cysteinylgl
Three novel alkoxyl-functionalized ionic liquids (ILs), 1-(3-triethoxysiyl propyl)-3-methyl imidazolium tetrafluoroborate (TESPMIM[BF4]), 1-(3-triethoxysiyl propyl)-3-methyl imidazolium hexafluorophos
Cytokinins (CTKs) are a class of growth regulating hormones that play a central role in a wide range of biological processes in plants.Many challenges have been faced to develop a sensitive and accura
Bi-template imprinted polymer has the ability to simultaneously separate and enrich two target molecules from one sample.Currently, there are few report involved in the application of bi-template prot
Molecular imprinting technology is a promising and facile method to produce molecular imprinted polymers (MIPs) with molecular-specific recognition sites to the target molecules.MIPs possess desired s
Molecular imprinting is a versatile and easy method to build selective recognition sites in a stable polymer matrix, and it is also an effective method for the preparation of selective sorbents.The mo
The objective of this paper is to propose a simple and effective pretreatment method for the enrichment of alkaloids.This pretreatment method was also applied to develop improved methods for the prepa
Glycan profiles on monoclonal antibodies (mAbs) impact the therapeutic efficacy.Glycosylation is affected by many factors such as cell line in which the mAb is produced as well as specific production