【摘 要】
：
Due to its large enhancement effect,nano-structure-based surface-enhanced Raman scattering(SERS)technology had been widely applied for bioanalysis and cell imaging.However,most SERS nanostructures suf
【机 构】
：
State Key Laboratory of Physical Chemistry of Solid Surfaces,the Key Laboratory for Chemical Biology
【出 处】
：
2016年分析化学前沿国际研讨会及中美分析化学研讨会
论文部分内容阅读
Due to its large enhancement effect,nano-structure-based surface-enhanced Raman scattering(SERS)technology had been widely applied for bioanalysis and cell imaging.However,most SERS nanostructures suffer from poor signal reproducibility,which hinders the application of SERS nanostructures in quantitative detection.Herein,we report an mPEG-SH-assisted approach to synthesize SERS-active plasmonic nanoparticles with one nm interior nanogap for multiplex quantitative detection and cancer cell imaging.Raman dyes were attached to a gold nanoparticle surface using mPEG-SH to prepare gold cores.Next,Ag atoms were deposited on gold cores in the presence of Pluronic F127 to form an Ag shell.HAuCl4 was then used to etch the Ag shell and form an interior nanogap in Au@AgAuNPs,leading to enhanced Raman intensity of dyes.SERS intensity distribution of Au@AgAuNPs was found to be more uniform than that of aggregated AuNPs.Finally,Au@AgAuNPs were used for multiplex quantitative detection and cancer cell imaging.Due to the advantages of simple and rapid preparation of Au@AgAuNPs with highly uniform,stable and reproducible Raman intensity,the method reported here will widen the applications of SERS-active nanoparticles in diagnostics and imaging.
其他文献
Colon adenocarcinoma is one of the most deadly human cancers due to its strong ability of invasion and metastasis.The development of diagnostic and therapeutic strategies against metastasis-related mo
Surface-enhanced Raman spectroscopy(SERS)has been developed as a versatile tool for trace-molecule detection and biomolecular analysis by coupled gold or silver nanostructures in the past two decades
The researches on the interactions between fluorophores and plasmonic structures have attracted many interests,which will not only be significant to understand the physical mechanisms of nano-optics,b
Two-dimensional sum-frequency generation(2D-SFG)spectroscopy with the surface selectivity provides many more observable information than one-dimensional SFG,such as cross-peaks,2D line shapes,anharmon
Many proteins contain cysteine residues,which play an important role in keeping the redox state,precise folding and stabilization of proteins.Consequently,evaluating the distribution of cysteine-conta
Sum frequency generation(SFG)spectroscopy is an ideal tool to study the surface/interface,due to the surface/interface selectivity and high detection sensitivity[1].In broadband SFG(BB-SFG),an infrare
Characterization of bacteria at a single-cell level is of great significance for basic biological studies and public health.However,flow cytometry as an efficient tool for single-cell analysis,is not
Recently,our group invented a new spectroscopic methodology called "shell-isolated nanoparticle-enhanced Raman spectroscopy"(SHINERS),which has been employed to overcome the long-term material-and sub
Thiol-disulfide exchanges are dynamic reactions that widely exist in organisms.Complex thiol-disulfide exchanges can take place on the cell surface due to the presence of many kinds of thiol-/disulfid
As with well-defined electronic levels and surface state,the single crystal surface obtained continual attentions from the surface science.Though SERS method is a powerful technique,it suffers with ma